Hotspot

petG-trnW-CCA

LSC · 68589-68709 · 121 bp

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Pi 0.0323
MarkerSeek score 54.40
Primer available yes
Label petG-trnW-CCA

Feature Metadata

label_name
petG-trnW-CCA
feature_type
igs
region
LSC
length_bp
121
variable_sites
8
pi
0.0323
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petG-trnW-CCA_p1 CAATACAGACGCGGTGATCA CAAAACCCGATGTCGTAGGT 50.0 50.0 58.1 57.9 189-193 bp 1.0 83.7
petG-trnW-CCA_p2 ACAATACAGACGCGGTGATC CAAAACCCGATGTCGTAGGT 50.0 50.0 58.1 57.9 190-194 bp 1.0 83.6
petG-trnW-CCA_p3 CAATACAGACGCGGTGATCA AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.1 58.0 225-229 bp 1.0 83.5
petG-trnW-CCA_p4 ACAATACAGACGCGGTGATC AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.1 58.0 226-230 bp 1.0 83.4
petG-trnW-CCA_p5 CGGTGATCAGTTGGACCTTT AGCGCTCTTAGTTCAGTTCG 50.0 50.0 57.8 58.0 214-218 bp 1.0 82.8