Hotspot

rbcL-accD

LSC · 58960-59754 · 795 bp

Back to Clivia

Pi 0.01
MarkerSeek score 44.00
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
795
variable_sites
20
pi
0.01
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 ACTACAGTTCGGTGGAGGAA CTTCCACCGGTATGTCCAAG 50.0 55.0 58.0 58.0 1130-1149 bp 1.0 83.3
rbcL-accD_p2 GCAGTAGCTAATCGGGTAGC CTTCCACCGGTATGTCCAAG 55.0 55.0 57.9 58.0 1078-1097 bp 1.0 83.0
rbcL-accD_p3 GAAGGGGAACGTGAGATGAC CTTCCACCGGTATGTCCAAG 55.0 55.0 58.0 58.0 1321-1340 bp 1.0 82.7
rbcL-accD_p4 GCGACAACGGTCTACTTCTT CTTCCACCGGTATGTCCAAG 50.0 55.0 57.9 58.0 1473-1492 bp 1.0 82.1
rbcL-accD_p5 CGGCGGTACAGTAGTAGGTA CTTCCACCGGTATGTCCAAG 55.0 55.0 58.0 58.0 1346-1365 bp 0.714286 71.0