Hotspot

ndhC-trnV-UAC

LSC · 52149-53747 · 1599 bp

Back to Clivia

Pi 0.00999
MarkerSeek score 50.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1599
variable_sites
35
pi
0.00999
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAGATAGGAATAGCACC AGAAGGTCTACGGTTCGAGT 47.6 50.0 57.5 57.8 1335-1730 bp 1.0 72.2
ndhC-trnV-UAC_p2 TGCCAAGATAGGAATAGCACC AGCCCAATGCAACAAAATGC 47.6 45.0 57.5 58.5 1417-1812 bp 1.0 69.9
ndhC-trnV-UAC_p3 GCCAAGATAGGAATAGCACCT AGAAGGTCTACGGTTCGAGT 47.6 50.0 57.2 57.8 1334-1729 bp 1.0 69.9
ndhC-trnV-UAC_p4 TGCCAAGATAGGAATAGCACC GAAGGTCTACGGTTCGAGTC 47.6 55.0 57.5 57.5 1334-1729 bp 1.0 69.5
ndhC-trnV-UAC_p5 TGCCAAGATAGGAATAGCACC GTTCGAGTCCGTATAGCCCT 47.6 55.0 57.5 58.7 1323-1718 bp 1.0 68.4