Hotspot

petN-psbM

LSC · 29553-30557 · 1005 bp

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Pi 0.00867
MarkerSeek score 43.60
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1005
variable_sites
20
pi
0.00867
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCTTGCTTGGGCTGCTTTAA AAAACAGTCAGCCAAAATGA 45.0 35.0 58.0 53.9 1105-1115 bp 1.0 49.9
petN-psbM_p2 TCTTGCTTGGGCTGCTTTAA AAAAACAGTCAGCCAAAATGA 45.0 33.3 58.0 54.8 1106-1116 bp 1.0 48.9
petN-psbM_p3 AGTAAGTCTTGCTTGGGCTG AAAACAGTCAGCCAAAATGA 50.0 35.0 57.8 53.9 1111-1121 bp 1.0 48.4
petN-psbM_p4 TCTTGCTTGGGCTGCTTTAA AAACAGTCAGCCAAAATGAT 45.0 35.0 58.0 53.6 1104-1114 bp 1.0 48.0
petN-psbM_p5 TCTTGCTTGGGCTGCTTTAA AACAGTCAGCCAAAATGATT 45.0 35.0 58.0 53.6 1103-1113 bp 1.0 48.0