Hotspot

psbI-trnS-GCU

LSC · 8299-8414 · 116 bp

Back to Clivia

Pi 0.035
MarkerSeek score 62.30
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
116
variable_sites
9
pi
0.035
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 CTTATCTAACGATCCGGGCC TAAAGCGGCGGATTGCTAAT 55.0 45.0 57.6 58.0 227-237 bp 1.0 80.2
psbI-trnS-GCU_p2 CTTATCTAACGATCCGGGCC GGGTTCGAATCCCTCTCTCT 55.0 55.0 57.6 58.2 182-192 bp 1.0 79.6
psbI-trnS-GCU_p3 CTTATCTAACGATCCGGGCC TTCGAATCCCTCTCTCTCCG 55.0 55.0 57.6 58.3 179-189 bp 1.0 78.9
psbI-trnS-GCU_p4 CTTATCTAACGATCCGGGCC GATGGCTGAGCGGACTAAAG 55.0 55.0 57.6 58.4 242-252 bp 1.0 76.9
psbI-trnS-GCU_p5 CTTATCTAACGATCCGGGCC CTAAAGCGGCGGATTGCTAA 55.0 50.0 57.6 58.7 228-238 bp 1.0 74.9