Hotspot

psbK-psbI

LSC · 7784-8187 · 404 bp

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Pi 0.00613
MarkerSeek score 43.20
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
404
variable_sites
7
pi
0.00613
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG GGCCCGGATCGTTAGATAAG 50.0 55.0 57.9 57.6 521-531 bp 1.0 78.0
psbK-psbI_p2 TGGCAAGCTGCTGTAAGTTT GGCCCGGATCGTTAGATAAG 45.0 55.0 58.3 57.6 518-528 bp 1.0 76.0
psbK-psbI_p3 TTGGCAAGCTGCTGTAAGTT GGCCCGGATCGTTAGATAAG 45.0 55.0 58.3 57.6 519-529 bp 1.0 76.0
psbK-psbI_p4 TTTGGCAAGCTGCTGTAAGT GGCCCGGATCGTTAGATAAG 45.0 55.0 58.3 57.6 520-530 bp 1.0 76.0
psbK-psbI_p5 GTTTGGCAAGCTGCTGTAAG TTTTTATTCCTCGCGTCCGG 50.0 50.0 57.9 58.6 547-557 bp 1.0 75.8