Hotspot

trnQ-UUG-psbK

LSC · 7260-7597 · 338 bp

Back to Clivia

Pi 0.0082
MarkerSeek score 35.20
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
LSC
length_bp
338
variable_sites
6
pi
0.0082
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
35.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 CCGTACACTCGATCACGTTT CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.0 57.9 757-759 bp 1.0 80.9
trnQ-UUG-psbK_p2 AACAAAAGGCGGGATCACAT CTTACAGCAGCTTGCCAAAC 45.0 50.0 58.1 57.9 675-677 bp 1.0 80.4
trnQ-UUG-psbK_p3 ACAAAAGGCGGGATCACATT CTTACAGCAGCTTGCCAAAC 45.0 50.0 58.1 57.9 674-676 bp 1.0 80.4
trnQ-UUG-psbK_p4 TCAGAACAAAAGGCGGGATC CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.2 57.9 679-681 bp 1.0 79.5
trnQ-UUG-psbK_p5 CCGTACACTCGATCACGTTT TGGAAGGTCAGAACGAGGAA 50.0 50.0 58.0 58.3 938-940 bp 1.0 79.0