Hotspot

rpl36-rps8

LSC · 79851-80323 · 473 bp

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Pi 0.103

MarkerSeek score 81.30

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 473
variable_sites: 45
pi: 0.103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	CGAACAGAGGCTCCTATCTTC	AGAATCGGCGGAGAAGTTTT	52.4	45.0	57.6	57.5	516-532 bp	1.0	76.2
rpl36-rps8_p2	CGAACAGAGGCTCCTATCTT	AGAATCGGCGGAGAAGTTTT	50.0	45.0	56.4	57.5	516-532 bp	1.0	74.6
rpl36-rps8_p3	CGAACAGAGGCTCCTATCTTC	TCGGCGGAGAAGTTTTATGT	52.4	45.0	57.6	57.2	512-528 bp	1.0	74.0
rpl36-rps8_p4	CGAACAGAGGCTCCTATCTT	TCGGCGGAGAAGTTTTATGT	50.0	45.0	56.4	57.2	512-528 bp	1.0	72.4
rpl36-rps8_p5	CGAACAGAGGCTCCTATCTTC	TCGGCGGAGAAGTTTTATGTT	52.4	42.9	57.6	58.0	512-528 bp	1.0	71.6