Hotspot

atpI-atpH

LSC · 47739-48589 · 851 bp

Back to Clitoria

Pi 0.0731

MarkerSeek score 82.20

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 851
variable_sites: 60
pi: 0.0731
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	GCTGCGGCTTATATAGGTGA	ATAACGGAAGCGGCAGAAAT	50.0	45.0	57.3	58.0	915-925 bp	1.0	81.6
atpI-atpH_p2	GCTGCGGCTTATATAGGTGA	AATAACGGAAGCGGCAGAAA	50.0	45.0	57.3	58.2	916-926 bp	1.0	80.4
atpI-atpH_p3	TAGGTGAATCCATGGAAGGC	ATAACGGAAGCGGCAGAAAT	50.0	45.0	57.0	58.0	902-912 bp	1.0	79.4
atpI-atpH_p4	GCTGCGGCTTATATAGGTGAA	ATAACGGAAGCGGCAGAAAT	47.6	45.0	58.0	58.0	915-925 bp	1.0	79.2
atpI-atpH_p5	TAGGTGAATCCATGGAAGGC	AATAACGGAAGCGGCAGAAA	50.0	45.0	57.0	58.2	903-913 bp	1.0	78.2