Hotspot

trnfM-CAU-trnG-GCC

LSC · 25353-25470 · 118 bp

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Pi 0.124

MarkerSeek score 81.10

Primer available yes

Label trnfM-CAU-trnG-GCC

Feature Metadata

label_name: trnfM-CAU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 118
variable_sites: 13
pi: 0.124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-trnG-GCC_p1	GCAATTTGGTAGCTCGCAAG	TCTTTGCCAAGGAGAAGACG	50.0	50.0	58.1	57.8	217-225 bp	1.0	86.5
trnfM-CAU-trnG-GCC_p2	GTAGCTCGCAAGGCTCATAA	TCTTTGCCAAGGAGAAGACG	50.0	50.0	57.8	57.8	209-217 bp	1.0	85.7
trnfM-CAU-trnG-GCC_p3	TAGCTCGCAAGGCTCATAAC	TCTTTGCCAAGGAGAAGACG	50.0	50.0	57.8	57.8	208-216 bp	1.0	85.7
trnfM-CAU-trnG-GCC_p4	AGGTTACGGGTTCAAATCCC	TCTTTGCCAAGGAGAAGACG	50.0	50.0	57.5	57.8	184-192 bp	1.0	84.2
trnfM-CAU-trnG-GCC_p5	AGCAATTTGGTAGCTCGCAA	TCTTTGCCAAGGAGAAGACG	45.0	50.0	58.5	57.8	218-226 bp	1.0	83.6