Hotspot

trnK-UUU-rbcL

LSC · 4718-5876 · 1159 bp

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Pi 0.134

MarkerSeek score 76.80

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 1159
variable_sites: 112
pi: 0.134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	CCTTTCTGGATCAGTCGTGG	AGTCCTGAATTAGCTGCTGC	55.0	50.0	58.0	58.0	1087-1361 bp	1.0	91.2
trnK-UUU-rbcL_p2	CCTTTCTGGATCAGTCGTGG	AATGAGGGACGTGATCTTGC	55.0	50.0	58.0	58.0	1147-1421 bp	1.0	90.9
trnK-UUU-rbcL_p3	CCTTTCTGGATCAGTCGTGG	GCCGTAGCTAATCGAGTAGC	55.0	55.0	58.0	58.0	1192-1466 bp	1.0	90.6
trnK-UUU-rbcL_p4	CCTTTCTGGATCAGTCGTGG	TGCTAGAGAATTGGGCGTTC	55.0	50.0	58.0	58.0	1947 bp	0.5	59.7
trnK-UUU-rbcL_p5	TGGACGAACCAATTGCTTCA	TGCTAGAGAATTGGGCGTTC	45.0	50.0	58.0	58.0	1904 bp	0.5	59.6