Hotspot

trnH-GUG-psbA

LSC · 146-661 · 516 bp

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Pi 0.214

MarkerSeek score 74.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 516
variable_sites: 76
pi: 0.214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GGTATCTGGTTTACCGCGTT	55.0	50.0	58.1	58.0	616-756 bp	1.0	90.9
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GGTATCTGGTTTACCGCGTT	50.0	50.0	57.9	58.0	630-770 bp	1.0	90.8
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	GGTATCTGGTTTACCGCGTT	50.0	50.0	57.9	58.0	629-769 bp	1.0	90.8
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	GGTATCTGGTTTACCGCGTT	50.0	50.0	57.9	58.0	631-771 bp	1.0	90.8
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CCAATTGGTCAGGGAAGCTT	55.0	50.0	58.1	58.1	976-1116 bp	1.0	88.9