Hotspot

ycf1

SSC · 121544-126994 · 5451 bp

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Pi 0.0179

MarkerSeek score 53.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5451
variable_sites: 281
pi: 0.0179
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTATCCCAGGCCTCTGCTAT	TGACGGGGTTGTCAGAAATG	55.0	50.0	58.1	58.1	1263 bp	1.0	84.1
ycf1_p2	TATCCCAGGCCTCTGCTATC	TGACGGGGTTGTCAGAAATG	55.0	50.0	58.1	58.1	1262 bp	1.0	84.1
ycf1_p3	CTATCCCAGGCCTCTGCTAT	GACGGGGTTGTCAGAAATGA	55.0	50.0	58.1	57.8	1262 bp	1.0	83.6
ycf1_p4	AAATCACTACACGTCTGCCC	TGACGGGGTTGTCAGAAATG	50.0	50.0	57.9	58.1	866 bp	0.75	72.8
ycf1_p5	GTACCAAAGCCACATCGTCT	TGACGGGGTTGTCAGAAATG	50.0	50.0	57.9	58.1	947 bp	0.5	61.3