Hotspot

trnT(UGU)-trnL(UAA)

LSC · 45859-46538 · 680 bp

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Pi 0.0176

MarkerSeek score 69.00

Primer available yes

Label trnT(UGU)-trnL(UAA)

Feature Metadata

label_name: trnT(UGU)-trnL(UAA)
feature_type: igs
region: LSC
length_bp: 680
variable_sites: 36
pi: 0.0176
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT(UGU)-trnL(UAA)_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	727-742 bp	1.0	84.1
trnT(UGU)-trnL(UAA)_p2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	759-774 bp	1.0	83.0
trnT(UGU)-trnL(UAA)_p3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	721-736 bp	1.0	82.9
trnT(UGU)-trnL(UAA)_p4	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	50.0	50.0	57.8	57.6	753-768 bp	1.0	81.7
trnT(UGU)-trnL(UAA)_p5	CTAACCTCTGAGCTAAGCGG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.5	58.2	719-734 bp	1.0	81.3