Hotspot

ycf3-trnS(GGA)

LSC · 43620-44442 · 823 bp

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Pi 0.0186

MarkerSeek score 64.90

Primer available yes

Label ycf3-trnS(GGA)

Feature Metadata

label_name: ycf3-trnS(GGA)
feature_type: igs
region: LSC
length_bp: 823
variable_sites: 44
pi: 0.0186
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS(GGA)_p1	GCTGTCCGTTGGTACTAGTG	CCCTCGGTAAACAAAAGCCT	55.0	50.0	58.0	58.1	1223-1302 bp	1.0	85.8
ycf3-trnS(GGA)_p2	GCTGTCCGTTGGTACTAGTG	TTCGATTGGGTATGGCTTCG	55.0	50.0	58.0	58.1	1854-1942 bp	1.0	85.0
ycf3-trnS(GGA)_p3	GCTGTCCGTTGGTACTAGTG	GGGGTCAACAGGTCAAGTTT	55.0	50.0	58.0	57.9	1913-2001 bp	1.0	84.7
ycf3-trnS(GGA)_p4	AGGTGTAGTGCTTCTTCCCT	TTCGATTGGGTATGGCTTCG	50.0	50.0	58.0	58.1	1877-1965 bp	0.875	79.4
ycf3-trnS(GGA)_p5	AGGTGTAGTGCTTCTTCCCT	GGGGTCAACAGGTCAAGTTT	50.0	50.0	58.0	57.9	1936-2024 bp	0.875	79.1