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psaA-ycf3

LSC · 40892-41674 · 783 bp

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Pi 0.0261

MarkerSeek score 72.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 783
variable_sites: 50
pi: 0.0261
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	875-921 bp	1.0	86.4
psaA-ycf3_p2	GAAAAATGACCCGGTTTGGC	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.2	57.9	893-939 bp	1.0	85.5
psaA-ycf3_p3	TTTTACTTCTGGTTCCGGCG	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.5	57.9	828-874 bp	1.0	83.8
psaA-ycf3_p4	TTTGGCCCATTCCTCGAAAG	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.5	57.9	879-925 bp	1.0	83.7
psaA-ycf3_p5	GGCCCATTCCTCGAAAGAAG	ATTGGTTGAAGATCACGGGG	55.0	50.0	58.6	57.9	876-922 bp	1.0	82.4