Hotspot

petN-psbM

LSC · 28064-29011 · 948 bp

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Pi 0.0179

MarkerSeek score 71.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 948
variable_sites: 48
pi: 0.0179
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACCGCTTTTCTACT	50.0	42.9	58.2	56.4	1089-1109 bp	1.0	63.4
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TCATTCTAGTTCCTACCGCT	50.0	45.0	58.2	55.4	1096-1116 bp	1.0	63.3
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TCATTCTAGTTCCTACCGCTT	50.0	42.9	58.2	56.2	1096-1116 bp	1.0	61.7
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TTCATTCTAGTTCCTACCGCT	50.0	42.9	58.2	56.2	1097-1117 bp	1.0	61.7
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACCGCTTTTCTACTT	50.0	40.9	58.2	57.1	1089-1109 bp	1.0	61.2