Hotspot

atpF-atpH

LSC · 12910-13176 · 267 bp

Back to Clinopodium

Pi 0.0227

MarkerSeek score 72.20

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 267
variable_sites: 16
pi: 0.0227
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTAGCCCAAAGAAACGA	CCGAGGCAGAGGGAAAAATA	50.0	50.0	57.8	57.3	427-439 bp	1.0	77.3
atpF-atpH_p2	TTAAATCCGAAACTCCCGGC	CCGAGGCAGAGGGAAAAATA	50.0	50.0	58.3	57.3	453-465 bp	1.0	76.4
atpF-atpH_p3	CCAGTAGCCCAAAGAAACGA	GGATTGGTTGTAGCACTAGCA	50.0	47.6	57.8	58.0	356-368 bp	1.0	75.8
atpF-atpH_p4	CCAGTAGCCCAAAGAAACGA	TGGATTGGTTGTAGCACTAGC	50.0	47.6	57.8	58.0	357-369 bp	1.0	75.8
atpF-atpH_p5	CCAGTAGCCCAAAGAAACGA	GCAGAGGGAAAAATACGAGGT	50.0	47.6	57.8	58.0	422-434 bp	1.0	75.0