Hotspot

psbI-trnS(GCU)

LSC · 8040-8125 · 86 bp

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Pi 0.0478

MarkerSeek score 75.00

Primer available yes

Label psbI-trnS(GCU)

Feature Metadata

label_name: psbI-trnS(GCU)
feature_type: igs
region: LSC
length_bp: 86
variable_sites: 10
pi: 0.0478
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS(GCU)_p1	ATCTAATGATCCGGGGCGTA	TTCGAATCCCTCTCTTTCCG	50.0	50.0	58.1	57.0	146-221 bp	1.0	78.2
psbI-trnS(GCU)_p2	TATCTAATGATCCGGGGCGT	TTCGAATCCCTCTCTTTCCG	50.0	50.0	58.1	57.0	147-222 bp	1.0	78.2
psbI-trnS(GCU)_p3	ATCTAATGATCCGGGGCGTA	GGGTTCGAATCCCTCTCTTT	50.0	50.0	58.1	56.9	149-224 bp	1.0	77.1
psbI-trnS(GCU)_p4	TATCTAATGATCCGGGGCGT	GGGTTCGAATCCCTCTCTTT	50.0	50.0	58.1	56.9	150-225 bp	1.0	77.1
psbI-trnS(GCU)_p5	ATCTAATGATCCGGGGCGTA	GGTTCGAATCCCTCTCTTTCC	50.0	52.4	58.1	58.1	148-223 bp	1.0	77.1