Hotspot

rps16-trnQ(UUG)

LSC · 5937-6898 · 962 bp

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Pi 0.0385

MarkerSeek score 81.10

Primer available yes

Label rps16-trnQ(UUG)

Feature Metadata

label_name: rps16-trnQ(UUG)
feature_type: igs
region: LSC
length_bp: 962
variable_sites: 87
pi: 0.0385
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ(UUG)_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	999-1084 bp	1.0	89.0
rps16-trnQ(UUG)_p2	AAGTCGCACGTTGCTTTCTA	GTCTCGCTATTCGGAGGTTC	45.0	55.0	58.1	57.9	1012-1097 bp	1.0	88.5
rps16-trnQ(UUG)_p3	AAGTCGCACGTTGCTTTCTA	CGGGTTTTGGTCTCGCTATT	45.0	50.0	58.1	58.3	1021-1106 bp	1.0	87.3
rps16-trnQ(UUG)_p4	AAGTCGCACGTTGCTTTCTA	TTTGGTCTCGCTATTCGGAG	45.0	50.0	58.1	57.4	1016-1101 bp	1.0	84.8
rps16-trnQ(UUG)_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1003-1088 bp	1.0	84.2