primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH(GUG)-psbA_p1	trnH(GUG)-psbA	1	ATCCACTTGGCTACATCCG	CTATCGAAGCTCCAACAAATG	19	21	52.6	42.9	56.9	55.0	0.0	0.0	6.047923	81	402	349-376	1.000000	50.0
trnH(GUG)-psbA_p2	trnH(GUG)-psbA	2	ATCCACTTGGCTACATCCGC	CTATCGAAGCTCCAACAAATG	20	21	55.0	42.9	60.2	55.0	0.0	0.0	6.135114	81	402	349-376	1.000000	50.0
trnH(GUG)-psbA_p3	trnH(GUG)-psbA	3	CCACTTGGCTACATCCGC	CTATCGAAGCTCCAACAAATG	18	21	61.1	42.9	58.2	55.0	0.0	0.0	6.142473	81	402	347-374	1.000000	50.0
trnH(GUG)-psbA_p4	trnH(GUG)-psbA	4	ATCCACTTGGCTACATCCG	TATCGAAGCTCCAACAAATG	19	20	52.6	40.0	56.9	53.7	0.0	0.0	6.380367	81	402	348-375	1.000000	50.0
trnH(GUG)-psbA_p5	trnH(GUG)-psbA	5	ATCCACTTGGCTACATCCGC	TATCGAAGCTCCAACAAATG	20	20	55.0	40.0	60.2	53.7	0.0	0.0	6.467559	81	402	348-375	1.000000	50.0
rps16-trnQ(UUG)_p1	rps16-trnQ(UUG)	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5937	6898	999-1084	1.000000	89.0
rps16-trnQ(UUG)_p2	rps16-trnQ(UUG)	2	AAGTCGCACGTTGCTTTCTA	GTCTCGCTATTCGGAGGTTC	20	20	45.0	55.0	58.1	57.9	8.1	0.0	0.261887	5937	6898	1012-1097	1.000000	88.5
rps16-trnQ(UUG)_p3	rps16-trnQ(UUG)	3	AAGTCGCACGTTGCTTTCTA	CGGGTTTTGGTCTCGCTATT	20	20	45.0	50.0	58.1	58.3	8.1	0.0	0.405161	5937	6898	1021-1106	1.000000	87.3
rps16-trnQ(UUG)_p4	rps16-trnQ(UUG)	4	AAGTCGCACGTTGCTTTCTA	TTTGGTCTCGCTATTCGGAG	20	20	45.0	50.0	58.1	57.4	8.1	0.0	0.718531	5937	6898	1016-1101	1.000000	84.8
rps16-trnQ(UUG)_p5	rps16-trnQ(UUG)	5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	5937	6898	1003-1088	1.000000	84.2
psbI-trnS(GCU)_p1	psbI-trnS(GCU)	1	ATCTAATGATCCGGGGCGTA	TTCGAATCCCTCTCTTTCCG	20	20	50.0	50.0	58.1	57.0	0.0	0.0	1.028505	8040	8125	146-221	1.000000	78.2
psbI-trnS(GCU)_p2	psbI-trnS(GCU)	2	TATCTAATGATCCGGGGCGT	TTCGAATCCCTCTCTTTCCG	20	20	50.0	50.0	58.1	57.0	0.0	0.0	1.028505	8040	8125	147-222	1.000000	78.2
psbI-trnS(GCU)_p3	psbI-trnS(GCU)	3	ATCTAATGATCCGGGGCGTA	GGGTTCGAATCCCTCTCTTT	20	20	50.0	50.0	58.1	56.9	0.0	17.2	1.149533	8040	8125	149-224	1.000000	77.1
psbI-trnS(GCU)_p4	psbI-trnS(GCU)	4	TATCTAATGATCCGGGGCGT	GGGTTCGAATCCCTCTCTTT	20	20	50.0	50.0	58.1	56.9	0.0	17.2	1.149533	8040	8125	150-225	1.000000	77.1
psbI-trnS(GCU)_p5	psbI-trnS(GCU)	5	ATCTAATGATCCGGGGCGTA	GGTTCGAATCCCTCTCTTTCC	20	21	50.0	52.4	58.1	58.1	0.0	0.0	1.159344	8040	8125	148-223	1.000000	77.1
atpF-atpH_p1	atpF-atpH	1	CCAGTAGCCCAAAGAAACGA	CCGAGGCAGAGGGAAAAATA	20	20	50.0	50.0	57.8	57.3	0.0	0.0	0.889008	12910	13176	427-439	1.000000	77.3
atpF-atpH_p2	atpF-atpH	2	TTAAATCCGAAACTCCCGGC	CCGAGGCAGAGGGAAAAATA	20	20	50.0	50.0	58.3	57.3	0.0	0.0	0.968568	12910	13176	453-465	1.000000	76.4
atpF-atpH_p3	atpF-atpH	3	CCAGTAGCCCAAAGAAACGA	GGATTGGTTGTAGCACTAGCA	20	21	50.0	47.6	57.8	58.0	0.0	0.0	1.193016	12910	13176	356-368	1.000000	75.8
atpF-atpH_p4	atpF-atpH	4	CCAGTAGCCCAAAGAAACGA	TGGATTGGTTGTAGCACTAGC	20	21	50.0	47.6	57.8	58.0	0.0	0.0	1.193016	12910	13176	357-369	1.000000	75.8
atpF-atpH_p5	atpF-atpH	5	CCAGTAGCCCAAAGAAACGA	GCAGAGGGAAAAATACGAGGT	20	21	50.0	47.6	57.8	58.0	0.0	0.0	1.191680	12910	13176	422-434	1.000000	75.0
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACCGCTTTTCTACT	20	21	50.0	42.9	58.2	56.4	0.0	0.0	2.762471	28064	29011	1089-1109	1.000000	63.4
petN-psbM_p2	petN-psbM	2	TAGTAAGTCTCGCTTGGGCT	TCATTCTAGTTCCTACCGCT	20	20	50.0	45.0	58.2	55.4	0.0	0.0	2.781070	28064	29011	1096-1116	1.000000	63.3
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	TCATTCTAGTTCCTACCGCTT	20	21	50.0	42.9	58.2	56.2	0.0	0.0	2.973013	28064	29011	1096-1116	1.000000	61.7
petN-psbM_p4	petN-psbM	4	TAGTAAGTCTCGCTTGGGCT	TTCATTCTAGTTCCTACCGCT	20	21	50.0	42.9	58.2	56.2	0.0	0.0	2.973013	28064	29011	1097-1117	1.000000	61.7
petN-psbM_p5	petN-psbM	5	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACCGCTTTTCTACTT	20	22	50.0	40.9	58.2	57.1	0.0	0.0	3.037844	28064	29011	1089-1109	1.000000	61.2
trnT(GGU)-psbD_p1	trnT(GGU)-psbD	1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	20	20	55.0	50.0	58.1	58.1	7.6	0.0	0.177181	30610	31968	1511-1535	1.000000	83.1
trnT(GGU)-psbD_p2	trnT(GGU)-psbD	2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.199336	30610	31968	1514-1538	1.000000	82.9
trnT(GGU)-psbD_p3	trnT(GGU)-psbD	3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	30610	31968	1501-1525	1.000000	82.0
trnT(GGU)-psbD_p4	trnT(GGU)-psbD	4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	30610	31968	1504-1528	1.000000	81.8
trnT(GGU)-psbD_p5	trnT(GGU)-psbD	5	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.395920	30610	31968	1488-1512	1.000000	81.4
trnG(GCC)-trnfM(CAU)_p1	trnG(GCC)-trnfM(CAU)	1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	58.1	58.0	0.0	4.2	0.096882	35602	35760	225-242	1.000000	85.4
trnG(GCC)-trnfM(CAU)_p2	trnG(GCC)-trnfM(CAU)	2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	58.1	58.0	0.0	4.2	0.096882	35602	35760	226-243	1.000000	85.4
trnG(GCC)-trnfM(CAU)_p3	trnG(GCC)-trnfM(CAU)	3	AGAAGATGCGGGTTCGATTC	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.084264	35602	35760	261-278	1.000000	84.7
trnG(GCC)-trnfM(CAU)_p4	trnG(GCC)-trnfM(CAU)	4	GAGAAGATGCGGGTTCGATT	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.084264	35602	35760	262-279	1.000000	84.7
trnG(GCC)-trnfM(CAU)_p5	trnG(GCC)-trnfM(CAU)	5	ATGCGGATATGGTCGAATGG	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.124730	35602	35760	302-319	1.000000	83.7
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	20	20	50.0	50.0	57.9	57.9	4.1	0.0	0.244113	40892	41674	875-921	1.000000	86.4
psaA-ycf3_p2	psaA-ycf3	2	GAAAAATGACCCGGTTTGGC	ATTGGTTGAAGATCACGGGG	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.338015	40892	41674	893-939	1.000000	85.5
psaA-ycf3_p3	psaA-ycf3	3	TTTTACTTCTGGTTCCGGCG	ATTGGTTGAAGATCACGGGG	20	20	50.0	50.0	58.5	57.9	0.0	0.0	0.613689	40892	41674	828-874	1.000000	83.8
psaA-ycf3_p4	psaA-ycf3	4	TTTGGCCCATTCCTCGAAAG	ATTGGTTGAAGATCACGGGG	20	20	50.0	50.0	58.5	57.9	0.0	0.0	0.584047	40892	41674	879-925	1.000000	83.7
psaA-ycf3_p5	psaA-ycf3	5	GGCCCATTCCTCGAAAGAAG	ATTGGTTGAAGATCACGGGG	20	20	55.0	50.0	58.6	57.9	0.0	0.0	0.745248	40892	41674	876-922	1.000000	82.4
ycf3-trnS(GGA)_p1	ycf3-trnS(GGA)	1	GCTGTCCGTTGGTACTAGTG	CCCTCGGTAAACAAAAGCCT	20	20	55.0	50.0	58.0	58.1	5.4	0.0	0.102193	43620	44442	1223-1302	1.000000	85.8
ycf3-trnS(GGA)_p2	ycf3-trnS(GGA)	2	GCTGTCCGTTGGTACTAGTG	TTCGATTGGGTATGGCTTCG	20	20	55.0	50.0	58.0	58.1	5.4	0.0	0.059726	43620	44442	1854-1942	1.000000	85.0
ycf3-trnS(GGA)_p3	ycf3-trnS(GGA)	3	GCTGTCCGTTGGTACTAGTG	GGGGTCAACAGGTCAAGTTT	20	20	55.0	50.0	58.0	57.9	5.4	0.0	0.075196	43620	44442	1913-2001	1.000000	84.7
ycf3-trnS(GGA)_p4	ycf3-trnS(GGA)	4	AGGTGTAGTGCTTCTTCCCT	TTCGATTGGGTATGGCTTCG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.083391	43620	44442	1877-1965	0.875000	79.4
ycf3-trnS(GGA)_p5	ycf3-trnS(GGA)	5	AGGTGTAGTGCTTCTTCCCT	GGGGTCAACAGGTCAAGTTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.098860	43620	44442	1936-2024	0.875000	79.1
trnT(UGU)-trnL(UAA)_p1	trnT(UGU)-trnL(UAA)	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	45859	46538	727-742	1.000000	84.1
trnT(UGU)-trnL(UAA)_p2	trnT(UGU)-trnL(UAA)	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	45859	46538	759-774	1.000000	83.0
trnT(UGU)-trnL(UAA)_p3	trnT(UGU)-trnL(UAA)	3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	45859	46538	721-736	1.000000	82.9
trnT(UGU)-trnL(UAA)_p4	trnT(UGU)-trnL(UAA)	4	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	57.8	57.6	15.5	0.0	0.651279	45859	46538	753-768	1.000000	81.7
trnT(UGU)-trnL(UAA)_p5	trnT(UGU)-trnL(UAA)	5	CTAACCTCTGAGCTAAGCGG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.5	58.2	5.5	10.2	0.748957	45859	46538	719-734	1.000000	81.3
ndhF_p1	ndhF	1	GAGAATCAGGACAATTCAAGA	TGACTCCTTTCAAAAGAAGT	21	20	38.1	35.0	53.3	52.2	0.0	4.1	11.524320	108373	110604	2321-2327	1.000000	43.8
ndhF_p2	ndhF	2	GAGAATCAGGACAATTCAAGA	TCCTTTCAAAAGAAGTAAAATCCGA	21	25	38.1	32.0	53.3	57.2	0.0	4.1	11.558869	108373	110604	2317-2323	1.000000	43.8
ndhF_p3	ndhF	3	GAGAATCAGGACAATTCAAGA	TTGACTCCTTTCAAAAGAAGT	21	21	38.1	33.3	53.3	53.2	0.0	4.1	11.564875	108373	110604	2322-2328	1.000000	43.8
ndhF_p4	ndhF	4	GAGAATCAGGACAATTCAAGA	CTCCTTTCAAAAGAAGTAAAATCCG	21	25	38.1	36.0	53.3	56.9	0.0	4.1	11.836664	108373	110604	2318-2324	1.000000	43.8
ndhF_p5	ndhF	5	GAGAATCAGGACAATTCAAGA	CCTTTCAAAAGAAGTAAAATCCGA	21	24	38.1	33.3	53.3	55.9	0.0	4.1	11.865498	108373	110604	2316-2322	1.000000	43.8
ndhF-rpl32_p1	ndhF-rpl32	1	GCCCACATACGACGAAGATT	ACCGGCCAGTCTTACTATGA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.218634	110605	111066	1111-1311	1.000000	87.3
ndhF-rpl32_p2	ndhF-rpl32	2	TCGGAAAAAGAAGAAGCCCC	ACCGGCCAGTCTTACTATGA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.248930	110605	111066	1080-1280	1.000000	87.2
ndhF-rpl32_p3	ndhF-rpl32	3	AAAAGCCCACATACGACGAA	ACCGGCCAGTCTTACTATGA	20	20	45.0	50.0	57.8	57.8	0.0	0.0	0.327418	110605	111066	1115-1315	1.000000	86.4
ndhF-rpl32_p4	ndhF-rpl32	4	ACACATCCCAACCAATTCCC	ACCGGCCAGTCTTACTATGA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.217376	110605	111066	1460-1660	0.875000	81.2
ndhF-rpl32_p5	ndhF-rpl32	5	CACATCCCAACCAATTCCCA	ACCGGCCAGTCTTACTATGA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.218768	110605	111066	1459-1659	0.875000	81.2
rpl32-trnL(UAG)_p1	rpl32-trnL(UAG)	1	TCGGAAAAAGAAGAAGCCCC	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.218640	111253	111954	1317-1517	1.000000	87.6
rpl32-trnL(UAG)_p2	rpl32-trnL(UAG)	2	TCGGAAAAAGAAGAAGCCCC	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.279367	111253	111954	1310-1510	1.000000	87.1
rpl32-trnL(UAG)_p3	rpl32-trnL(UAG)	3	TCGGAAAAAGAAGAAGCCCC	ACTGAAGGGCGGGTACTAAT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.218874	111253	111954	1858-2063	1.000000	86.6
rpl32-trnL(UAG)_p4	rpl32-trnL(UAG)	4	TCGGAAAAAGAAGAAGCCCC	GATGATATGCCATGCCGCTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.122782	111253	111954	1370-1556	0.875000	82.8
rpl32-trnL(UAG)_p5	rpl32-trnL(UAG)	5	CTGGAAGTGGAACGAAAGGT	GATGATATGCCATGCCGCTA	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.284537	111253	111954	1331-1517	0.875000	81.6
ccsA-ndhD_p1	ccsA-ndhD	1	TTGGAGTTGGGACCCAAAAG	GGTTGATAATAGTGGGCGCA	20	20	50.0	50.0	57.9	58.0	27.9	10.8	0.118860	113120	113348	1111-1138	1.000000	84.0
ccsA-ndhD_p2	ccsA-ndhD	2	TTGGAGTTGGGACCCAAAAG	TGGTTGATAATAGTGGGCGC	20	20	50.0	50.0	57.9	58.0	27.9	0.0	0.118860	113120	113348	1112-1139	1.000000	84.0
ccsA-ndhD_p3	ccsA-ndhD	3	CTAACGAAGCATGGGGATCC	GGTTGATAATAGTGGGCGCA	20	20	55.0	50.0	58.1	58.0	1.9	10.8	0.153971	113120	113348	1133-1160	1.000000	83.7
ccsA-ndhD_p4	ccsA-ndhD	4	CTAACGAAGCATGGGGATCC	TGGTTGATAATAGTGGGCGC	20	20	55.0	50.0	58.1	58.0	1.9	0.0	0.153971	113120	113348	1134-1161	1.000000	83.7
ccsA-ndhD_p5	ccsA-ndhD	5	ATTAGTACCCGCCCTTCAGT	GGTTGATAATAGTGGGCGCA	20	20	50.0	50.0	58.1	58.0	0.0	10.8	0.162718	113120	113348	1498-1525	1.000000	83.2
ndhD_p1	ndhD	1	GAATTAGTGAGAGCCCCGTG	TGATACCTTGGGATGGGTGT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.078866	113349	114878	1946-1946	0.125000	44.4
ndhD_p2	ndhD	2	GTGAATTAGTGAGAGCCCCG	TGATACCTTGGGATGGGTGT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.078866	113349	114878	1948-1948	0.125000	44.4
ndhD_p3	ndhD	3	TTGGAGTTGGGACCCAAAAG	TGATACCTTGGGATGGGTGT	20	20	50.0	50.0	57.9	58.0	27.9	0.0	0.100845	113349	114878	2230-2230	0.125000	44.2
ndhD_p4	ndhD	4	CTAACGAAGCATGGGGATCC	TGATACCTTGGGATGGGTGT	20	20	55.0	50.0	58.1	58.0	1.9	0.0	0.135955	113349	114878	2252-2252	0.125000	43.9
ndhD_p5	ndhD	5	GAATTAGTGAGAGCCCCGTG	ATGTACCCAATGTGTCCGTG	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.166625	113349	114878	1990-1990	0.125000	43.7
rps15-ycf1_p1	rps15-ycf1	1	ACCCTCTATTTTCTTCGTTTTCT	TCCGCGATTGAAATTTTGTGA	23	21	34.8	38.1	55.9	57.1	0.0	8.2	7.047774	121133	121543	492-536	1.000000	49.2
rps15-ycf1_p2	rps15-ycf1	2	GACCCTCTATTTTCTTCGTTTTCT	TCCGCGATTGAAATTTTGTGA	24	21	37.5	38.1	57.0	57.1	0.0	8.2	6.952507	121133	121543	493-537	1.000000	49.1
rps15-ycf1_p3	rps15-ycf1	3	GACCCTCTATTTTCTTCGTT	TCCGCGATTGAAATTTTGTGA	20	21	40.0	38.1	52.6	57.1	0.0	8.2	7.273255	121133	121543	493-537	1.000000	49.1
rps15-ycf1_p4	rps15-ycf1	4	GACCCTCTATTTTCTTCGTTTTCT	TCCGCGATTGAAATTTTGTGAA	24	22	37.5	36.4	57.0	57.8	0.0	8.2	7.276807	121133	121543	493-537	1.000000	49.1
rps15-ycf1_p5	rps15-ycf1	5	GACCCTCTATTTTCTTCGTTTTC	TCCGCGATTGAAATTTTGTGA	23	21	39.1	38.1	55.6	57.1	0.0	8.2	7.298327	121133	121543	493-537	1.000000	49.1
ycf1_p1	ycf1	1	CTATCCCAGGCCTCTGCTAT	TGACGGGGTTGTCAGAAATG	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.159325	125267	125386	1263-1263	1.000000	84.1
ycf1_p2	ycf1	2	TATCCCAGGCCTCTGCTATC	TGACGGGGTTGTCAGAAATG	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.164947	125267	125386	1262-1262	1.000000	84.1
ycf1_p3	ycf1	3	CTATCCCAGGCCTCTGCTAT	GACGGGGTTGTCAGAAATGA	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.229113	125267	125386	1262-1262	1.000000	83.6
ycf1_p4	ycf1	4	AAATCACTACACGTCTGCCC	TGACGGGGTTGTCAGAAATG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.203886	125267	125386	866-866	0.750000	72.8
ycf1_p5	ycf1	5	GTACCAAAGCCACATCGTCT	TGACGGGGTTGTCAGAAATG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.203886	125267	125386	947-947	0.500000	61.3