Hotspot

rps3

LSC · 81672-82334 · 663 bp

Back to Climacoptera

Pi 0.0166

MarkerSeek score 55.20

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 663
variable_sites: 11
pi: 0.0166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TCCATCGTTCCCATCGTTTC	GTCTCATTCCACGCGATGTA	50.0	50.0	58.0	57.8	1465-1468 bp	1.0	79.5
rps3_p2	TCCATCGTTCCCATCGTTTC	TCTCATTCCACGCGATGTAC	50.0	50.0	58.0	57.8	1464-1467 bp	1.0	79.5
rps3_p3	CCATCGTTCCCATCGTTTCT	GTCTCATTCCACGCGATGTA	50.0	50.0	58.0	57.8	1464-1467 bp	1.0	79.4
rps3_p4	CCATCGTTCCCATCGTTTCT	TCTCATTCCACGCGATGTAC	50.0	50.0	58.0	57.8	1463-1466 bp	1.0	79.4
rps3_p5	TCCATCGTTCCCATCGTTTC	CTCATTCCACGCGATGTACT	50.0	50.0	58.0	57.8	1463-1466 bp	1.0	79.4