Pi
0.0952
MarkerSeek score
72.00
Primer available
yes
Label
rpl23
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rpl23_p1 | ACGTCCCATAATAGGTCCCA | TGAAACTCGAACTCATAGGGA | 50.0 | 42.9 | 57.5 | 56.1 | 224-254 bp | 1.0 | 62.4 |
| rpl23_p2 | ACGTCCCATAATAGGTCCCA | ACTCGAACTCATAGGGAAGA | 50.0 | 45.0 | 57.5 | 55.0 | 220-250 bp | 1.0 | 61.5 |
| rpl23_p3 | ACGTCCCATAATAGGTCCCA | TGAAACTCGAACTCATAGGGAAG | 50.0 | 43.5 | 57.5 | 57.9 | 224-254 bp | 1.0 | 60.9 |
| rpl23_p4 | ACGTCCCATAATAGGTCCCA | TGAAACTCGAACTCATAGGGAA | 50.0 | 40.9 | 57.5 | 56.8 | 224-254 bp | 1.0 | 60.3 |
| rpl23_p5 | ACGTCCCATAATAGGTCCCA | TTGAAACTCGAACTCATAGGGA | 50.0 | 40.9 | 57.5 | 56.8 | 225-255 bp | 1.0 | 60.3 |
| rpl23_p1 | GAATGGTTAAAGCGCCCAAC | GTTCGCCCGTTATGGATAGT | 50.0 | 50.0 | 58.0 | 57.5 | 277 bp | 0.5 | 55.8 |
| rpl23_p2 | GGAGCGAAACAATCAGCCTA | GTTCGCCCGTTATGGATAGT | 50.0 | 50.0 | 58.0 | 57.5 | 795 bp | 0.5 | 55.6 |
| rpl23_p3 | GAATGGTTAAAGCGCCCAAC | CGTTCGCCCGTTATGGATAG | 50.0 | 55.0 | 58.0 | 58.6 | 278 bp | 0.5 | 55.2 |
| rpl23_p4 | CCAAATTCGGGGGTCAAAGA | GTTCGCCCGTTATGGATAGT | 50.0 | 50.0 | 58.1 | 57.5 | 551 bp | 0.5 | 55.2 |
| rpl23_p5 | TCCAAATTCGGGGGTCAAAG | GTTCGCCCGTTATGGATAGT | 50.0 | 50.0 | 58.1 | 57.5 | 552 bp | 0.5 | 55.2 |