Hotspot

rpl23-trnH-GUG

IRa · 150041-150057 · 17 bp

Back to Climacoptera

Pi 0.176
MarkerSeek score 72.60
Primer available yes
Label rpl23-trnH-GUG

Feature Metadata

label_name
rpl23-trnH-GUG
feature_type
igs
region
IRa
length_bp
17
variable_sites
3
pi
0.176
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-trnH-GUG_p1 GAATGGTTAAAGCGCCCAAC GGGTTCAATTCCCGTCGTT 50.0 52.6 58.0 58.1 293 bp 0.5 51.4
rpl23-trnH-GUG_p2 CCAAATTCGGGGGTCAAAGA GGGTTCAATTCCCGTCGTT 50.0 52.6 58.1 58.1 567 bp 0.5 50.8
rpl23-trnH-GUG_p3 CTCCAAATTCGGGGGTCAAA GGGTTCAATTCCCGTCGTT 50.0 52.6 58.1 58.1 569 bp 0.5 50.8
rpl23-trnH-GUG_p4 TCCAAATTCGGGGGTCAAAG GGGTTCAATTCCCGTCGTT 50.0 52.6 58.1 58.1 568 bp 0.5 50.8
rpl23-trnH-GUG_p5 GAATGGTTAAAGCGCCCAAC GGGTTCAATTCCCGTCGTTC 50.0 55.0 58.0 59.2 293 bp 0.5 50.3