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rps4-trnT-UGU

LSC · 45480-45906 · 427 bp

Back to Climacoptera

Pi 0.0342

MarkerSeek score 69.40

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 427
variable_sites: 14
pi: 0.0342
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AGTCCAGGTAAAGCCCCTAG	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.1	57.9	544-552 bp	1.0	82.7
rps4-trnT-UGU_p2	GTCCAGGTAAAGCCCCTAGA	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.1	57.9	543-551 bp	1.0	82.6
rps4-trnT-UGU_p3	TCCAGGTAAAGCCCCTAGAC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.1	57.9	542-550 bp	1.0	82.6
rps4-trnT-UGU_p4	AGTCCAGGTAAAGCCCCTAG	ATGGTCATCGGTTCGATTCC	55.0	50.0	58.1	57.8	512-520 bp	1.0	82.1
rps4-trnT-UGU_p5	AGCCCCTAGACGACGTATTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.2	57.9	533-541 bp	1.0	81.9