Hotspot

ycf1

SSC · 126916-132549 · 5634 bp

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Pi 0.00213

MarkerSeek score 41.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5634
variable_sites: 12
pi: 0.00213
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCCCTTTTTGCTTTCTATTGA	AGATCAAGAAATCAATTCACCCA	36.4	34.8	56.1	56.1	243 bp	1.0	40.7
ycf1_p2	ACCCCTTTTTGCTTTCTATTGATG	AGATCAAGAAATCAATTCACCCA	37.5	34.8	58.0	56.1	243 bp	1.0	40.7
ycf1_p3	CCCCTTTTTGCTTTCTATTGATGT	AGATCAAGAAATCAATTCACCCA	37.5	34.8	58.0	56.1	242 bp	1.0	40.7
ycf1_p4	TCGATGATATAACCCCTTTTTGC	AGATCAAGAAATCAATTCACCCA	39.1	34.8	57.0	56.1	254 bp	1.0	40.6
ycf1_p5	CGATGATATAACCCCTTTTTGCT	AGATCAAGAAATCAATTCACCCA	39.1	34.8	57.0	56.1	253 bp	1.0	40.6