Hotspot

rpl16

LSC · 84055-85527 · 1473 bp

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Pi 0.00343
MarkerSeek score 47.80
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1473
variable_sites
5
pi
0.00343
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 AGCATATCGGCGATTGCTAG AAATTGCAGGGCGTATCGAT 50.0 45.0 58.0 58.0 1957-1976 bp 1.0 80.9
rpl16_p2 CATATCGGCGATTGCTAGCT AAATTGCAGGGCGTATCGAT 50.0 45.0 58.0 58.0 1955-1974 bp 1.0 80.9
rpl16_p3 GAGCATATCGGCGATTGCTA AAATTGCAGGGCGTATCGAT 50.0 45.0 58.0 58.0 1958-1977 bp 1.0 80.9
rpl16_p4 CAAATGGGTCTGGGGTTGAA AAATTGCAGGGCGTATCGAT 50.0 45.0 58.0 58.0 1882-1901 bp 1.0 80.9
rpl16_p5 TTCAAATGGGTCTGGGGTTG AAATTGCAGGGCGTATCGAT 50.0 45.0 58.0 58.0 1884-1903 bp 1.0 80.9