Hotspot

petD

LSC · 79280-80551 · 1272 bp

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Pi 0.00236

MarkerSeek score 50.70

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1272
variable_sites: 3
pi: 0.00236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	AGTTGAAGGGAATTCTCAGAAGA	TCCCTAGATACACACGTCGT	39.1	50.0	57.2	57.6	1367 bp	1.0	46.2
petD_p2	GTTGAAGGGAATTCTCAGAAGA	TCCCTAGATACACACGTCGT	40.9	50.0	55.7	57.6	1366 bp	1.0	42.8
petD_p3	AGTTGAAGGGAATTCTCAGAAG	TCCCTAGATACACACGTCGT	40.9	50.0	55.7	57.6	1367 bp	1.0	42.7
petD_p4	AGTTGAAGGGAATTCTCAGAAGAT	TCCCTAGATACACACGTCGT	37.5	50.0	57.6	57.6	1367 bp	1.0	41.8
petD_p5	AGTTGAAGGGAATTCTCAGA	TCCCTAGATACACACGTCGT	40.0	50.0	53.5	57.6	1367 bp	1.0	41.3