Hotspot

petB

LSC · 77645-79072 · 1428 bp

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Pi 0.0035

MarkerSeek score 55.60

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 5
pi: 0.0035
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	ACCGCAAAGTCCTAGCTTTC	TGTTCCTCCCCCAAATGATG	50.0	50.0	58.2	57.5	1603 bp	1.0	74.5
petB_p2	GAACCGCAAAGTCCTAGCTT	TGTTCCTCCCCCAAATGATG	50.0	50.0	58.2	57.5	1605 bp	1.0	74.5
petB_p3	CCGCAAAGTCCTAGCTTTCA	TGTTCCTCCCCCAAATGATG	50.0	50.0	58.2	57.5	1602 bp	1.0	74.5
petB_p4	AACCGCAAAGTCCTAGCTTT	TGTTCCTCCCCCAAATGATG	45.0	50.0	57.7	57.5	1604 bp	1.0	73.8
petB_p5	TAGTTCGACCGCGGAATTTC	TGTTCCTCCCCCAAATGATG	50.0	50.0	58.4	57.5	1527 bp	1.0	73.1