Hotspot

rpl16-rps3

LSC · 85528-85644 · 117 bp

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Pi 0.0265

MarkerSeek score 71.00

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 117
variable_sites: 3
pi: 0.0265
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	TGCGAAGTGATGAGTTGGTT	CCCTACAAACCATTCGAGCT	45.0	50.0	57.7	57.6	304-323 bp	1.0	79.5
rpl16-rps3_p2	AGTTCATACTACGGGGGCTT	CCCTACAAACCATTCGAGCT	50.0	50.0	58.1	57.6	281-285 bp	1.0	77.8
rpl16-rps3_p3	TCATACTACGGGGGCTTTGA	CCCTACAAACCATTCGAGCT	50.0	50.0	58.1	57.6	278-282 bp	1.0	77.7
rpl16-rps3_p4	AGTTCATACTACGGGGGCTT	GGGTTCCCCTACAAACCATT	50.0	50.0	58.1	57.4	287-291 bp	1.0	76.0
rpl16-rps3_p5	AGTTCATACTACGGGGGCTT	CCCCTACAAACCATTCGAGC	50.0	55.0	58.1	58.6	282-286 bp	1.0	76.0