Hotspot

psaJ-rpl33

LSC · 69872-70326 · 455 bp

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Pi 0.00659

MarkerSeek score 50.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 455
variable_sites: 3
pi: 0.00659
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TACTCTGTGGTTCGGGTCTT	GGACATCTTTACCCTTGGCC	50.0	55.0	58.0	58.2	568 bp	1.0	78.5
psaJ-rpl33_p2	ATGCGTTGACATTCCCCTTT	GGACATCTTTACCCTTGGCC	45.0	55.0	58.1	58.2	509 bp	1.0	78.0
psaJ-rpl33_p3	TGTGGCACCGGTACTAAGTA	GGACATCTTTACCCTTGGCC	50.0	55.0	58.1	58.2	586 bp	1.0	77.9
psaJ-rpl33_p4	CATATCTCTCTGTGGCACCG	GGACATCTTTACCCTTGGCC	55.0	55.0	57.9	58.2	596 bp	1.0	77.3
psaJ-rpl33_p5	TGGCACCGGTACTAAGTACT	GGACATCTTTACCCTTGGCC	50.0	55.0	57.8	58.2	584 bp	1.0	76.8