Hotspot

accD-psaI

LSC · 61160-61897 · 738 bp

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Pi 0.00275

MarkerSeek score 61.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 738
variable_sites: 2
pi: 0.00275
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTCCACGCTTTCTTTCCTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.8	58.0	839-851 bp	1.0	75.0
accD-psaI_p2	GCTCCACGCTTTCTTTCCTT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.8	57.9	845-857 bp	1.0	74.4
accD-psaI_p3	GCTCCACGCTTTCTTTCCT	CCATTGCAATTGCCGGAAAT	52.6	45.0	58.1	58.0	839-851 bp	1.0	72.6
accD-psaI_p4	TCCACGCTTTCTTTCCTTTGA	CCATTGCAATTGCCGGAAAT	42.9	45.0	58.1	58.0	837-849 bp	1.0	72.5
accD-psaI_p5	GCTCCACGCTTTCTTTCCT	AAGAAGCCATTGCAATTGCC	52.6	45.0	58.1	57.9	845-857 bp	1.0	72.0