Hotspot

ycf3-trnS-GGA

LSC · 46433-46763 · 331 bp

Back to Clethra

Pi 0.00906

MarkerSeek score 56.30

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 331
variable_sites: 3
pi: 0.00906
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	GCGAAGGTACGGAAAGAGAG	45.0	55.0	56.4	58.1	523-524 bp	1.0	67.1
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	GCGAAGGTACGGAAAGAGAG	45.0	55.0	56.4	58.1	524-525 bp	1.0	67.1
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	494-495 bp	1.0	67.1
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	495-496 bp	1.0	67.1
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	515-516 bp	1.0	66.6