Hotspot

ccsA

SSC · 112659-113612 · 954 bp

Back to Clerodendrum

Pi 0.0119

MarkerSeek score 53.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 954
variable_sites: 27
pi: 0.0119
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TGGCGGCATATCATCTCCTA	TCAGTAGCTTTTCGATGGCC	50.0	50.0	58.1	58.0	1683-1695 bp	1.0	82.9
ccsA_p2	TGGCGGCATATCATCTCCTA	CAGTAGCTTTTCGATGGCCT	50.0	50.0	58.1	58.0	1682-1694 bp	1.0	82.9
ccsA_p3	TAGACACGCTGCTCTTAGGA	TCAGTAGCTTTTCGATGGCC	50.0	50.0	57.9	58.0	1736-1748 bp	1.0	82.3
ccsA_p4	TAGACACGCTGCTCTTAGGA	CAGTAGCTTTTCGATGGCCT	50.0	50.0	57.9	58.0	1735-1747 bp	1.0	82.3
ccsA_p5	AACCTAAATTGGCCTGGCTC	TCAGTAGCTTTTCGATGGCC	50.0	50.0	58.1	58.0	2191-2200 bp	1.0	82.3