Hotspot

petA-psbJ

LSC · 62850-63734 · 885 bp

Back to Clerodendrum

Pi 0.0191

MarkerSeek score 68.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 885
variable_sites: 39
pi: 0.0191
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	GCCGATACTACTGGAAGGAT	50.0	50.0	58.2	56.2	1037-1062 bp	1.0	67.9
petA-psbJ_p2	TCGAGAAGGTTCAATTGGCC	GCCGATACTACTGGAAGGAT	50.0	50.0	58.2	56.2	1039-1064 bp	1.0	67.9
petA-psbJ_p3	AGAAGGTTCAATTGGCCGAA	GCCGATACTACTGGAAGGAT	45.0	50.0	57.7	56.2	1036-1061 bp	1.0	67.0
petA-psbJ_p4	CCCATTACGTGTCCAAGGTC	GCCGATACTACTGGAAGGAT	55.0	50.0	58.3	56.2	1122-1147 bp	1.0	66.9
petA-psbJ_p5	GACCCATTACGTGTCCAAGG	GCCGATACTACTGGAAGGAT	55.0	50.0	58.3	56.2	1124-1149 bp	1.0	66.9