Hotspot

ndhC-trnV-UAC

LSC · 50328-51186 · 859 bp

Back to Clerodendrum

Pi 0.0156
MarkerSeek score 66.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
859
variable_sites
31
pi
0.0156
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAAATAGGAATAAGACTTGA AGAAGGTCTACGGTTCGAGT 33.3 50.0 56.3 57.8 942-971 bp 1.0 46.6
ndhC-trnV-UAC_p2 TGCCAAAATAGGAATAAGACTTGA GAAGGTCTACGGTTCGAGTC 33.3 55.0 56.3 57.5 941-970 bp 1.0 46.6
ndhC-trnV-UAC_p3 TGCCAAAATAGGAATAAGACTTG AGAAGGTCTACGGTTCGAGT 34.8 50.0 54.9 57.8 942-971 bp 1.0 46.6
ndhC-trnV-UAC_p4 TGCCAAAATAGGAATAAGACTTGA GTTCGAGTCCGTATAGCCCT 33.3 55.0 56.3 58.7 930-959 bp 1.0 46.6
ndhC-trnV-UAC_p5 TGCCAAAATAGGAATAAGACTTGAT AGAAGGTCTACGGTTCGAGT 32.0 50.0 56.8 57.8 942-971 bp 1.0 46.6