Hotspot

rps2-rpoC2

LSC · 16591-16802 · 212 bp

Back to Clerodendrum

Pi 0.0415

MarkerSeek score 70.70

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 212
variable_sites: 18
pi: 0.0415
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CCGCATCTCTTTTCGGAAGA	GGGTCGAATCGATTGGTTGA	50.0	50.0	58.0	58.0	875-881 bp	1.0	83.4
rps2-rpoC2_p2	TCTTCCCGCTTTTTGTTCCA	GGGTCGAATCGATTGGTTGA	45.0	50.0	57.9	58.0	847-853 bp	1.0	83.0
rps2-rpoC2_p3	CCGCATCTCTTTTCGGAAGA	AAGGGTGCTGGTTTCAGAAG	50.0	50.0	58.0	58.0	1094-1100 bp	1.0	82.8
rps2-rpoC2_p4	CCGCATCTCTTTTCGGAAGA	GAACTGATTGGGTTGTTGCG	50.0	50.0	58.0	57.9	1045-1051 bp	1.0	82.6
rps2-rpoC2_p5	TCTTCCCGCTTTTTGTTCCA	AAGGGTGCTGGTTTCAGAAG	45.0	50.0	57.9	58.0	1066-1072 bp	1.0	82.4