Hotspot

trnS-GCU-trnG-UCC

LSC · 8680-9321 · 642 bp

Back to Clerodendrum

Pi 0.0261

MarkerSeek score 66.60

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 642
variable_sites: 37
pi: 0.0261
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	709-736 bp	1.0	56.6
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	708-735 bp	1.0	56.6
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.3	712-739 bp	1.0	55.5
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	711-738 bp	1.0	55.5
trnS-GCU-trnG-UCC_p5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	710-737 bp	1.0	55.5