Hotspot

trnK-UUU-rps16

LSC · 4266-4925 · 660 bp

Back to Clerodendrum

Pi 0.0178

MarkerSeek score 62.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 660
variable_sites: 27
pi: 0.0178
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TCAGTCGCGGTCTTACAAAG	GCCGTACGAGGAGAAAACTT	50.0	50.0	57.9	57.9	976-1090 bp	0.8	77.4
trnK-UUU-rps16_p2	TCAGTCGCGGTCTTACAAAG	TCTATCCCAATGAGCCGTCT	50.0	50.0	57.9	57.9	920-1031 bp	0.8	77.2
trnK-UUU-rps16_p3	TCAGTCGCGGTCTTACAAAG	AAGATCTTCGGAAAGTGGGC	50.0	50.0	57.9	57.9	858-969 bp	0.8	77.1
trnK-UUU-rps16_p4	TCAGTCGCGGTCTTACAAAG	GATCTTCGGAAAGTGGGCTT	50.0	50.0	57.9	57.9	856-967 bp	0.8	77.1
trnK-UUU-rps16_p5	TCAGTCGCGGTCTTACAAAG	AGCCGTCTATCGAATCGTTG	50.0	50.0	57.9	57.9	908-1019 bp	0.8	77.0