primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GTCGTGGGTATCTGGTTCAC	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.134890	76	386	527-589	1.000000	90.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	CGTGGGTATCTGGTTCACTG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.139065	76	386	525-587	1.000000	90.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	GTCGTGGGTATCTGGTTCAC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.139252	76	386	541-603	1.000000	90.1
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	GTCGTGGGTATCTGGTTCAC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.140760	76	386	540-602	1.000000	90.1
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	GAGTGGGAGCTTAGTTTCCG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.140460	76	386	977-1039	1.000000	87.2
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	TCAGTCGCGGTCTTACAAAG	GCCGTACGAGGAGAAAACTT	20	20	50.0	50.0	57.9	57.9	6.1	18.8	0.136305	4266	4925	976-1090	0.800000	77.4
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	TCAGTCGCGGTCTTACAAAG	TCTATCCCAATGAGCCGTCT	20	20	50.0	50.0	57.9	57.9	6.1	0.0	0.148430	4266	4925	920-1031	0.800000	77.2
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCAGTCGCGGTCTTACAAAG	AAGATCTTCGGAAAGTGGGC	20	20	50.0	50.0	57.9	57.9	6.1	0.9	0.175668	4266	4925	858-969	0.800000	77.1
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TCAGTCGCGGTCTTACAAAG	GATCTTCGGAAAGTGGGCTT	20	20	50.0	50.0	57.9	57.9	6.1	0.0	0.175668	4266	4925	856-967	0.800000	77.1
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCAGTCGCGGTCTTACAAAG	AGCCGTCTATCGAATCGTTG	20	20	50.0	50.0	57.9	57.9	6.1	1.5	0.181442	4266	4925	908-1019	0.800000	77.0
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6083	7330	1258-1339	1.000000	86.0
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6083	7330	1262-1343	1.000000	81.3
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.7	8.1	0.0	0.818817	6083	7330	1288-1369	1.000000	81.1
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.2	58.1	0.0	16.9	0.863824	6083	7330	1250-1331	1.000000	80.8
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AGTCGCACGTTGCTTTCTAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.9	58.1	8.1	16.9	0.938588	6083	7330	1257-1338	1.000000	80.2
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	GTAATCCTGGACGCGAAGAA	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	57.7	58.0	15.9	0.0	0.293543	8476	8591	204-219	1.000000	83.7
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	GTAATCCTGGACGCGAAGAA	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	57.7	58.0	15.9	0.0	0.294931	8476	8591	205-220	1.000000	83.7
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	GTAATCCTGGACGCGAAGAA	TTAGCTTGGGAGAGATGGCT	20	20	50.0	50.0	57.7	58.1	15.9	0.0	0.403912	8476	8591	227-242	1.000000	82.2
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	GTAATCCTGGACGCGAAGAA	GACTAAAGCGTCGGATTGCT	20	20	50.0	50.0	57.7	58.4	15.9	2.3	0.647384	8476	8591	202-217	1.000000	80.9
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	GTAATCCTGGACGCGAAGAA	CTGAGTGGACTAAAGCGTCG	20	20	50.0	55.0	57.7	58.4	15.9	0.0	0.670100	8476	8591	209-224	1.000000	80.5
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	8680	9321	709-736	1.000000	56.6
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	8680	9321	708-735	1.000000	56.6
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8680	9321	712-739	1.000000	55.5
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8680	9321	711-738	1.000000	55.5
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.674529	8680	9321	710-737	1.000000	55.5
atpH-atpI_p1	atpH-atpI	1	AACGGAAGCAGCAGAAATCA	TTTTGCAACTTTAGCCGCAG	20	20	45.0	45.0	58.1	57.9	0.0	0.0	0.236520	13923	14971	836-1134	1.000000	84.7
atpH-atpI_p2	atpH-atpI	2	AGCCAATCCAGCAGCAATAA	TTTTGCAACTTTAGCCGCAG	20	20	45.0	45.0	57.9	57.9	0.0	0.0	0.281186	13923	14971	854-1152	1.000000	84.3
atpH-atpI_p3	atpH-atpI	3	GCCAATCCAGCAGCAATAAC	TTTTGCAACTTTAGCCGCAG	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.347127	13923	14971	853-1151	1.000000	83.7
atpH-atpI_p4	atpH-atpI	4	TCCAGCAGCAATAACGGAAG	TTTTGCAACTTTAGCCGCAG	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.394902	13923	14971	848-1146	1.000000	83.4
atpH-atpI_p5	atpH-atpI	5	ATCCAGCAGCAATAACGGAA	TTTTGCAACTTTAGCCGCAG	20	20	45.0	45.0	57.6	57.9	0.0	0.0	0.551108	13923	14971	849-1147	1.000000	82.1
rps2-rpoC2_p1	rps2-rpoC2	1	CCGCATCTCTTTTCGGAAGA	GGGTCGAATCGATTGGTTGA	20	20	50.0	50.0	58.0	58.0	0.0	17.0	0.028687	16591	16802	875-881	1.000000	83.4
rps2-rpoC2_p2	rps2-rpoC2	2	TCTTCCCGCTTTTTGTTCCA	GGGTCGAATCGATTGGTTGA	20	20	45.0	50.0	57.9	58.0	0.0	17.0	0.078592	16591	16802	847-853	1.000000	83.0
rps2-rpoC2_p3	rps2-rpoC2	3	CCGCATCTCTTTTCGGAAGA	AAGGGTGCTGGTTTCAGAAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023263	16591	16802	1094-1100	1.000000	82.8
rps2-rpoC2_p4	rps2-rpoC2	4	CCGCATCTCTTTTCGGAAGA	GAACTGATTGGGTTGTTGCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.060622	16591	16802	1045-1051	1.000000	82.6
rps2-rpoC2_p5	rps2-rpoC2	5	TCTTCCCGCTTTTTGTTCCA	AAGGGTGCTGGTTTCAGAAG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.073168	16591	16802	1066-1072	1.000000	82.4
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	CACTACGAGCGAAAGGGAAA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.156675	28452	29019	633-682	1.000000	84.6
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	ACGAGCGAAAGGGAAAATGT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.189918	28452	29019	629-678	1.000000	84.3
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	TACCATTAAAGCAGCCCACG	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.336896	28452	29019	603-652	1.000000	83.2
trnC-GCA-petN_p4	trnC-GCA-petN	4	GGGGGACTGCAAATCCTTTT	CACTACGAGCGAAAGGGAAA	20	20	50.0	50.0	58.4	57.9	14.7	0.0	0.430038	28452	29019	651-700	1.000000	82.2
trnC-GCA-petN_p5	trnC-GCA-petN	5	GGGGGACTGCAAATCCTTTT	ACGAGCGAAAGGGAAAATGT	20	20	50.0	45.0	58.4	58.1	14.7	0.0	0.463281	28452	29019	647-696	1.000000	82.0
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	57.9	58.2	0.0	0.0	0.349607	31544	32709	1324-1330	1.000000	80.0
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GCCCTTTTAACTCAGCGGTA	CAAAAACGAAACGGTCCCTC	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.498082	31544	32709	1323-1329	1.000000	78.9
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GGCGTAAGTCATCGGTTCAA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.3	58.2	0.0	0.0	0.528876	31544	32709	1287-1293	1.000000	78.7
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GCCCTTTTAACTCAGCGGTA	TCCCTCCGTAACCAGTCATC	20	20	50.0	55.0	57.9	58.5	0.0	0.0	0.621666	31544	32709	1309-1315	1.000000	77.9
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GGCGTAAGTCATCGGTTCAA	CAAAAACGAAACGGTCCCTC	20	20	50.0	50.0	58.3	57.6	0.0	0.0	0.677352	31544	32709	1286-1292	1.000000	77.5
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	46389	47072	733-772	1.000000	82.7
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGTTCCGGGGTTTCTCTGA	20	20	55.0	50.0	57.9	57.7	0.0	0.0	0.446811	46389	47072	807-846	1.000000	81.7
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	46389	47072	765-804	1.000000	81.6
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	46389	47072	727-766	1.000000	81.5
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	TAGTTCCGGGGTTTCTCTGA	20	20	50.0	50.0	57.8	57.7	15.5	0.0	0.554564	46389	47072	839-878	1.000000	80.6
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TGCCAAAATAGGAATAAGACTTGA	AGAAGGTCTACGGTTCGAGT	24	20	33.3	50.0	56.3	57.8	0.0	0.0	5.853835	50328	51186	942-971	1.000000	46.6
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGCCAAAATAGGAATAAGACTTGA	GAAGGTCTACGGTTCGAGTC	24	20	33.3	55.0	56.3	57.5	0.0	0.0	6.197608	50328	51186	941-970	1.000000	46.6
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TGCCAAAATAGGAATAAGACTTG	AGAAGGTCTACGGTTCGAGT	23	20	34.8	50.0	54.9	57.8	0.0	0.0	6.253715	50328	51186	942-971	1.000000	46.6
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGCCAAAATAGGAATAAGACTTGA	GTTCGAGTCCGTATAGCCCT	24	20	33.3	55.0	56.3	58.7	0.0	0.0	6.339901	50328	51186	930-959	1.000000	46.6
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGCCAAAATAGGAATAAGACTTGAT	AGAAGGTCTACGGTTCGAGT	25	20	32.0	50.0	56.8	57.8	0.0	0.0	6.395612	50328	51186	942-971	1.000000	46.6
petA-psbJ_p1	petA-psbJ	1	GAGAAGGTTCAATTGGCCGA	GCCGATACTACTGGAAGGAT	20	20	50.0	50.0	58.2	56.2	5.4	0.0	1.972289	62850	63734	1037-1062	1.000000	67.9
petA-psbJ_p2	petA-psbJ	2	TCGAGAAGGTTCAATTGGCC	GCCGATACTACTGGAAGGAT	20	20	50.0	50.0	58.2	56.2	5.4	0.0	1.972289	62850	63734	1039-1064	1.000000	67.9
petA-psbJ_p3	petA-psbJ	3	AGAAGGTTCAATTGGCCGAA	GCCGATACTACTGGAAGGAT	20	20	45.0	50.0	57.7	56.2	5.4	0.0	2.079870	62850	63734	1036-1061	1.000000	67.0
petA-psbJ_p4	petA-psbJ	4	CCCATTACGTGTCCAAGGTC	GCCGATACTACTGGAAGGAT	20	20	55.0	50.0	58.3	56.2	0.0	0.0	2.059343	62850	63734	1122-1147	1.000000	66.9
petA-psbJ_p5	petA-psbJ	5	GACCCATTACGTGTCCAAGG	GCCGATACTACTGGAAGGAT	20	20	55.0	50.0	58.3	56.2	0.0	0.0	2.059343	62850	63734	1124-1149	1.000000	66.9
rps19-rpl2_p1	rps19-rpl2	1	TGATTGGCTACAAAAGGATT	GATTGTTCGTCGCCGTAGTA	20	20	35.0	50.0	52.7	57.8	0.0	0.0	5.475257	83435	83494	119-126	1.000000	46.0
rps19-rpl2_p2	rps19-rpl2	2	TGATTGGCTACAAAAGGATT	CACAACCCCTTGGGGTTATC	20	20	35.0	55.0	52.7	58.2	0.0	35.0	5.449454	83435	83494	188-195	1.000000	44.2
rps19-rpl2_p3	rps19-rpl2	3	TGATTGGCTACAAAAGGATTT	CACAACCCCTTGGGGTTATC	21	20	33.3	55.0	53.6	58.2	0.0	35.0	5.509160	83435	83494	188-195	1.000000	44.2
rps19-rpl2_p4	rps19-rpl2	4	TGATTGGCTACAAAAGGATT	CGAGCCCCAATTGGTAGAAA	20	20	35.0	50.0	52.7	57.9	0.0	22.9	5.419566	83435	83494	214-221	1.000000	43.7
rps19-rpl2_p5	rps19-rpl2	5	TGATTGGCTACAAAAGGATTT	CGAGCCCCAATTGGTAGAAA	21	20	33.3	50.0	53.6	57.9	0.0	22.9	5.479272	83435	83494	214-221	1.000000	43.7
ndhF_p1	ndhF	1	ATACCGTCGCCCCTTTTTAC	AACTTGTCAAACTCGGCACA	20	20	50.0	45.0	58.0	58.3	0.0	0.0	0.283188	109061	111286	2917-2955	1.000000	80.8
ndhF_p2	ndhF	2	ATACCGTCGCCCCTTTTTAC	ACTTGTCAAACTCGGCACAA	20	20	50.0	45.0	58.0	58.3	0.0	0.0	0.283188	109061	111286	2916-2954	1.000000	80.8
ndhF_p3	ndhF	3	ACAGGAACAAGAGGGATCCA	AACTTGTCAAACTCGGCACA	20	20	50.0	45.0	58.0	58.3	15.2	0.0	0.299561	109061	111286	2804-2842	1.000000	80.8
ndhF_p4	ndhF	4	ACAGGAACAAGAGGGATCCA	ACTTGTCAAACTCGGCACAA	20	20	50.0	45.0	58.0	58.3	15.2	0.0	0.299561	109061	111286	2803-2841	1.000000	80.8
ndhF_p5	ndhF	5	AAGAGGGATCCACCGAAGAA	ACTTGTCAAACTCGGCACAA	20	20	50.0	45.0	58.0	58.3	15.2	0.0	0.302147	109061	111286	2797-2833	0.800000	72.3
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	TAGACACGCTGCTCTTAGGA	TGAAAGGGTCGAAAAAGAAA	20	20	50.0	35.0	57.9	53.2	0.0	0.0	4.885121	112554	112658	211-223	1.000000	46.4
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	TAGACACGCTGCTCTTAGGA	TTGAAAGGGTCGAAAAAGAA	20	20	50.0	35.0	57.9	53.2	0.0	0.0	4.885121	112554	112658	212-224	1.000000	46.4
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	AAATTGGTAGACACGCTGCT	TGAAAGGGTCGAAAAAGAAA	20	20	45.0	35.0	57.8	53.2	0.0	0.0	4.945848	112554	112658	218-230	1.000000	45.8
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	AAATTGGTAGACACGCTGCT	TTGAAAGGGTCGAAAAAGAA	20	20	45.0	35.0	57.8	53.2	0.0	0.0	4.945848	112554	112658	219-231	1.000000	45.8
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	GTAGACACGCTGCTCTTAGG	TGAAAGGGTCGAAAAAGAAA	20	20	55.0	35.0	57.8	53.2	0.0	0.0	4.957138	112554	112658	212-224	1.000000	45.8
ccsA_p1	ccsA	1	TGGCGGCATATCATCTCCTA	TCAGTAGCTTTTCGATGGCC	20	20	50.0	50.0	58.1	58.0	8.0	0.0	0.086985	112659	113612	1683-1695	1.000000	82.9
ccsA_p2	ccsA	2	TGGCGGCATATCATCTCCTA	CAGTAGCTTTTCGATGGCCT	20	20	50.0	50.0	58.1	58.0	8.0	3.3	0.092647	112659	113612	1682-1694	1.000000	82.9
ccsA_p3	ccsA	3	TAGACACGCTGCTCTTAGGA	TCAGTAGCTTTTCGATGGCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.148453	112659	113612	1736-1748	1.000000	82.3
ccsA_p4	ccsA	4	TAGACACGCTGCTCTTAGGA	CAGTAGCTTTTCGATGGCCT	20	20	50.0	50.0	57.9	58.0	0.0	3.3	0.154115	112659	113612	1735-1747	1.000000	82.3
ccsA_p5	ccsA	5	AACCTAAATTGGCCTGGCTC	TCAGTAGCTTTTCGATGGCC	20	20	50.0	50.0	58.1	58.0	19.7	0.0	0.171543	112659	113612	2191-2200	1.000000	82.3
ycf1_p1	ycf1	1	GCTATTCTCTTGCGTGGTGA	ATATATGGCAAACCCGACCG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.189057	122671	122790	957-957	0.800000	73.7
ycf1_p2	ycf1	2	AGCTATTCTCTTGCGTGGTG	ATATATGGCAAACCCGACCG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.194648	122671	122790	958-958	0.800000	73.6
ycf1_p3	ycf1	3	CAGCTATTCTCTTGCGTGGT	ATATATGGCAAACCCGACCG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.194648	122671	122790	959-959	0.800000	73.6
ycf1_p4	ycf1	4	GCTATTCTCTTGCGTGGTGA	GCAAACCCGACCGATAGAAA	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.280861	122671	122790	950-950	0.800000	73.0
ycf1_p5	ycf1	5	AGCTATTCTCTTGCGTGGTG	GCAAACCCGACCGATAGAAA	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.286452	122671	122790	951-951	0.800000	72.9