Hotspot

rpoC2

LSC · 16257-20414 · 4158 bp

Back to Cleomella

Pi 0.00192

MarkerSeek score 41.60

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4158
variable_sites: 8
pi: 0.00192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CGATTTCTTCGCCTCTTTGC	ACCCTTTCTTTCCGTCGAAA	50.0	45.0	57.8	57.4	222 bp	1.0	74.4
rpoC2_p2	CGATTTCTTCGCCTCTTTGC	ACATACCCTTTCTTTCCGTCG	50.0	47.6	57.8	58.0	226 bp	1.0	71.1
rpoC2_p3	CGATTTCTTCGCCTCTTTGC	CATACCCTTTCTTTCCGTCGA	50.0	47.6	57.8	57.8	225 bp	1.0	69.5
rpoC2_p4	CGATTTCTTCGCCTCTTTGC	TACCCTTTCTTTCCGTCGAA	50.0	45.0	57.8	56.5	223 bp	1.0	67.4
rpoC2_p5	TTCTTCGCCTCTTTGCTACT	ACCCTTTCTTTCCGTCGAAA	45.0	45.0	56.9	57.4	218 bp	1.0	66.7