Hotspot

rpl16

LSC · 80677-82243 · 1567 bp

Back to Cleomella

Pi 0.00323

MarkerSeek score 61.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1567
variable_sites: 5
pi: 0.00323
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCTTTTAATGCAAAGGACGAAGT	TCAGCAATTCTACAATTCTATAAGGT	34.8	30.8	57.0	56.5	1719-1735 bp	1.0	41.0
rpl16_p2	CTCTTCTTTTAATGCAAAGGACGA	TCAGCAATTCTACAATTCTATAAGGT	37.5	30.8	57.8	56.5	1723-1739 bp	1.0	41.0
rpl16_p3	TCTCTTCTTTTAATGCAAAGGACG	TCAGCAATTCTACAATTCTATAAGGT	37.5	30.8	57.8	56.5	1724-1740 bp	1.0	41.0
rpl16_p4	TCTTCTTTTAATGCAAAGGACGA	TCAGCAATTCTACAATTCTATAAGGT	34.8	30.8	56.7	56.5	1722-1738 bp	1.0	41.0
rpl16_p5	TCTTTTAATGCAAAGGACGAAGT	CAGCAATTCTACAATTCTATAAGGT	34.8	32.0	57.0	55.2	1718-1734 bp	1.0	41.0