Hotspot

psaC

SSC · 116883-117128 · 246 bp

Back to Cleomella

Pi 0.00407

MarkerSeek score 46.20

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 1
pi: 0.00407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	TGAGTACGAGTCCTTGTCAA	AGCAAATTTCTGGTAAAGGAAATCA	45.0	32.0	55.9	57.7	429 bp	1.0	40.2
psaC_p2	TTGAGTACGAGTCCTTGTCA	AGCAAATTTCTGGTAAAGGAAATCA	45.0	32.0	55.9	57.7	430 bp	1.0	40.2
psaC_p3	TGAGTACGAGTCCTTGTCAA	AGCAAATTTCTGGTAAAGGA	45.0	35.0	55.9	52.6	429 bp	1.0	40.2
psaC_p4	TTGAGTACGAGTCCTTGTCA	AGCAAATTTCTGGTAAAGGA	45.0	35.0	55.9	52.6	430 bp	1.0	40.2
psaC_p5	TGAGTACGAGTCCTTGTCAA	AGCAAATTTCTGGTAAAGGAA	45.0	33.3	55.9	53.6	429 bp	1.0	40.2