Hotspot

cemA

LSC · 60258-60947 · 690 bp

Back to Cleomella

Pi 0.00435

MarkerSeek score 61.20

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 3
pi: 0.00435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	TCAACAAATGAGGTAGGTTCA	AGAAGGAATCCCGCATAGTT	38.1	45.0	54.8	56.3	839 bp	1.0	40.3
cemA_p2	ATCAACAAATGAGGTAGGTTCA	AGAAGGAATCCCGCATAGTT	36.4	45.0	55.4	56.3	840 bp	1.0	40.3
cemA_p3	TCAACAAATGAGGTAGGTTCAT	AGAAGGAATCCCGCATAGTT	36.4	45.0	55.4	56.3	839 bp	1.0	40.3
cemA_p4	GAATCAACAAATGAGGTAGGTTCA	AGAAGGAATCCCGCATAGTT	37.5	45.0	57.2	56.3	842 bp	1.0	40.3
cemA_p5	CAACAAATGAGGTAGGTTCA	AGAAGGAATCCCGCATAGTT	40.0	45.0	53.1	56.3	838 bp	1.0	40.3