Hotspot

ndhC-trnV-GUA

LSC · 50065-50767 · 703 bp

Back to Cleomella

Pi 0.00427
MarkerSeek score 61.20
Primer available yes
Label ndhC-trnV-GUA

Feature Metadata

label_name
ndhC-trnV-GUA
feature_type
igs
region
LSC
length_bp
703
variable_sites
3
pi
0.00427
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-GUA_p1 TTTCTCTGGCCCCTTCCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 856-870 bp 1.0 78.3
ndhC-trnV-GUA_p2 TCTCTGGCCCCTTCCTAATT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 854-868 bp 1.0 76.4
ndhC-trnV-GUA_p3 TTCTCTGGCCCCTTCCTAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 855-869 bp 1.0 76.4
ndhC-trnV-GUA_p4 CTCTGGCCCCTTCCTAATTG AGAAGGTCTACGGTTCGAGT 55.0 50.0 57.6 57.8 853-867 bp 1.0 75.9
ndhC-trnV-GUA_p5 TTTCTCTGGCCCCTTCCTAA GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 855-869 bp 1.0 75.5