Hotspot

trnT-ACC-psbD

LSC · 31286-32400 · 1115 bp

Back to Cleomella

Pi 0.00269

MarkerSeek score 59.20

Primer available yes

Label trnT-ACC-psbD

Feature Metadata

label_name: trnT-ACC-psbD
feature_type: igs
region: LSC
length_bp: 1115
variable_sites: 3
pi: 0.00269
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-ACC-psbD_p1	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	55.0	45.0	58.1	58.0	1269-1271 bp	1.0	79.3
trnT-ACC-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1272-1274 bp	1.0	79.2
trnT-ACC-psbD_p3	CAGGAACTAAGTCAACCGGG	ACCTACAAAAACGAAGCGGT	55.0	45.0	57.9	58.0	1302-1304 bp	1.0	79.1
trnT-ACC-psbD_p4	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	55.0	50.0	58.1	57.7	1279-1281 bp	1.0	77.6
trnT-ACC-psbD_p5	GGCGTAAGTCATCGGTTCAA	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.3	58.0	1246-1248 bp	1.0	77.6