Hotspot

atpH-atpI

LSC · 13603-14333 · 731 bp

Back to Cleomella

Pi 0.00547

MarkerSeek score 61.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 731
variable_sites: 4
pi: 0.00547
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CAGGTCCAATGGAAGCAAGT	TAGCTGCGGCGTATATAGGT	50.0	50.0	58.1	58.2	848 bp	1.0	78.3
atpH-atpI_p2	AACCGAAGCAGCAGAAATCA	TAGCTGCGGCGTATATAGGT	45.0	50.0	58.1	58.2	805 bp	1.0	78.2
atpH-atpI_p3	CAGGTCCAATGGAAGCAAGT	CGTTAGCTGCGGCGTATATA	50.0	50.0	58.1	57.7	851 bp	1.0	77.7
atpH-atpI_p4	AACCGAAGCAGCAGAAATCA	CGTTAGCTGCGGCGTATATA	45.0	50.0	58.1	57.7	808 bp	1.0	77.6
atpH-atpI_p5	GGAAGCAAGTCCAACAGCTA	TAGCTGCGGCGTATATAGGT	50.0	50.0	57.8	58.2	838 bp	1.0	77.5