Hotspot

trnS-AGC-trnG-GGA

LSC · 8020-8725 · 706 bp

Back to Cleomella

Pi 0.00426

MarkerSeek score 61.50

Primer available yes

Label trnS-AGC-trnG-GGA

Feature Metadata

label_name: trnS-AGC-trnG-GGA
feature_type: igs
region: LSC
length_bp: 706
variable_sites: 3
pi: 0.00426
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-AGC-trnG-GGA_p1	CCAACGCTTTAGTCCACTCA	TGAATCAAACCGAGAGACCC	50.0	50.0	57.8	57.2	814-816 bp	1.0	73.1
trnS-AGC-trnG-GGA_p2	AACGCTTTAGTCCACTCAGC	TGAATCAAACCGAGAGACCC	50.0	50.0	58.2	57.2	812-814 bp	1.0	72.8
trnS-AGC-trnG-GGA_p3	GCTTTAGTCCACTCAGCCAT	TGAATCAAACCGAGAGACCC	50.0	50.0	57.6	57.2	809-811 bp	1.0	71.1
trnS-AGC-trnG-GGA_p4	TCCAACGCTTTAGTCCACTC	TGAATCAAACCGAGAGACCC	50.0	50.0	57.5	57.2	815-817 bp	1.0	70.8
trnS-AGC-trnG-GGA_p5	CCAACGCTTTAGTCCACTCA	GAATCAAACCGAGAGACCCT	50.0	50.0	57.8	56.9	813-815 bp	1.0	70.7