Hotspot

ndhF

SSC · 129706-131973 · 2268 bp

Pi 0.44

MarkerSeek score 79.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2268
variable_sites: 835
pi: 0.44
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CCATTTAGGTTTGAAATAGCGTGA	CCACCGAAGAAGCTCCTTTT	37.5	50.0	57.8	58.1	2551 bp	0.5	25.7
ndhF_p2	TCCATTTAGGTTTGAAATAGCGTG	CCACCGAAGAAGCTCCTTTT	37.5	50.0	57.8	58.1	2552 bp	0.5	25.7
ndhF_p3	CCATTTAGGTTTGAAATAGCGTGA	TATCCACCGAAGAAGCTCCT	37.5	50.0	57.8	57.8	2554 bp	0.5	25.2
ndhF_p4	TCCATTTAGGTTTGAAATAGCGTG	TATCCACCGAAGAAGCTCCT	37.5	50.0	57.8	57.8	2555 bp	0.5	25.2
ndhF_p5	CCATTTAGGTTTGAAATAGCGTGA	ACCGAAGAAGCTCCTTTTCC	37.5	50.0	57.8	57.8	2549 bp	0.5	25.0