Hotspot

ndhD

SSC · 124784-126226 · 1443 bp

Pi 0.489

MarkerSeek score 79.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1443
variable_sites: 567
pi: 0.489
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TCTGCTCCAAGAACAGAGGA	CCATCGGCATTCTTTCAGGA	50.0	50.0	58.0	58.0	2294 bp	0.5	59.5
ndhD_p2	AAACAACTCGAAGCATGGGT	CCATCGGCATTCTTTCAGGA	45.0	50.0	58.0	58.0	2200 bp	0.5	59.5
ndhD_p3	GGCATGAAACAACTCGAAGC	CCATCGGCATTCTTTCAGGA	50.0	50.0	58.0	58.0	2206 bp	0.5	59.4
ndhD_p4	AACTCGCATCAACCAATCGA	CCATCGGCATTCTTTCAGGA	45.0	50.0	57.9	58.0	2694 bp	0.5	58.9
ndhD_p5	TGCTTCTGCTCCAAGAACAG	CCATCGGCATTCTTTCAGGA	50.0	50.0	58.1	58.0	2298 bp	0.5	58.8